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Chai Lab

Center for Craniofacial Molecular Biology

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Chai Lab

Protocols

Sample processing and sectioning

  • Paraffin sample processing for mouse embryonic heads
  • Paraffin sample processing for mouse incisors
  • Frozen sample processing for mouse embryonic heads
  • Frozen sample processing for mouse incisors
  • Paraffin sample sectioning and mounting using slide warmer
  • Frozen sample sectioning and mounting with anti-roll plate

Tissue staining

  • Hematoxylin-Eosin staining
  • LacZ Staining on Cryosections

Immunofluorescence/Immunohistochemistry

  • Whole mount immunofluorescence on embryonic tissue slices
  • Immunofluorescence for paraffin-embedded tissue sections
  • Immunofluorescence on mouse incisor cryosections
  • Immunohistochemistry on cryosections using TSA Plus kits

RNAscope

  • RNAscope on cryosections using chromogenic substrate
  • Dual signal RNAscope on cryosections using chromogenic substrate

Western blot

  • Western blots for mouse incisor mesenchymal cells

Featured publications

News

  • New Manuscript Accepted in Nature Communications
  • New Publications in Development and Journal of Dental Research
  • New publications in eLife and Disease Models & Mechanisms
  • New publication: Embryonic requirements for Tcf12 in the development of the mouse coronal suture
  • New publication: Inhibiting Hh signaling in Gli1+ osteogenic progenitors alleviates TMJOA

Open positions

  • Postdoc position available

Links

  • Center for Craniofacial Molecular Biology
  • C-DOCTOR
  • FaceBase
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