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Center for Craniofacial Molecular Biology

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Chai Lab

Protocols

Sample processing and sectioning

  • Paraffin sample processing for mouse embryonic heads
  • Paraffin sample processing for mouse incisors
  • Frozen sample processing for mouse embryonic heads
  • Frozen sample processing for mouse incisors
  • Paraffin sample sectioning and mounting using slide warmer
  • Frozen sample sectioning and mounting with anti-roll plate

Tissue staining

  • Hematoxylin-Eosin staining
  • LacZ Staining on Cryosections

Immunofluorescence/Immunohistochemistry

  • Whole mount immunofluorescence on embryonic tissue slices
  • Immunofluorescence for paraffin-embedded tissue sections
  • Immunofluorescence on mouse incisor cryosections
  • Immunohistochemistry on cryosections using TSA Plus kits

RNAscope

  • RNAscope on cryosections using chromogenic substrate
  • Dual signal RNAscope on cryosections using chromogenic substrate

Western blot

  • Western blots for mouse incisor mesenchymal cells

Featured publications

Cover of Cell journal
Cover of Cell Biology Publication
Cover of Development journal April 2000
Cover of Cell Stem Cell
Cover of Developmental Cell
Generation of tamoxifen-inducible Tfap2b-CreERT2 mice using CRISPR-Cas9
Cover of Human Molecular Genetics
Cover of Development journal
Cover of Development journal November 2015
Cover of The Journal of Clinical Investigation
Cover of Development journal February 2014
Cover of Development journal May 2012
Cover of Developmental Cell April 2015
Cover of Developmental Biology 2015
Cover of Journal of Dental Research
Cover of Cell Reports
Cover of Science Advances
Cover of Development journal

News

  • New Publication in Nature Communications
  • New Publication in Proceedings of the National Academy of Sciences
  • New Publication in Nature Communications
  • New Publication Accepted in The Journal of Bone and Mineral Research
  • New Publication in Bone Research

Open positions

  • Postdoc position available

Links

  • Center for Craniofacial Molecular Biology
  • C-DOCTOR
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